Article: ‘De verwachte richtlijn duurzame corporate governance: verantwoord ondernemen moet hoog op de bestuursagenda’ in: Maandblad voor Ondernemingsrecht 2021/7. The spindle length, spindle volume (tubulin staining signal) and metaphase plate volume (DAPI signal) of MII eggs (isolated 17 h after hCG injection) were quantified using three-dimensional spindle reconstruction from confocal optical sections. In summarizing the expression of transposable element groups, each read was counted only once using a custom R script. e, MYSERV and related RLTR31B2 LTR-derived transcripts are upregulated in Mov10l1 –/– testes at 9 d. Mov10l1 –/– germ cells are indicated (asterisk), which show deviation from the normal DDX4 staining pattern in which strong cytoplasmic staining would surround a nucleus with a minimal signal.

Normalization: ACM uses normalization algorithms to weigh several types of evidence for merging and splitting names. First, only the reads with aligned first nucleotide were selected (all reads with a clipped 5′ end were removed).Although we observed changes in Mov10l1 –/– oocytes, which possibly contribute to the sterile phenotype, the mechanism of the loss of developmental competence of Mov10l1 –/– oocytes remains to be further delineated.

The post-meiotic and post-zygotic sterility observed in female Mov10l1 –/– hamsters adds to the repertoire of critical roles of the mammalian piRNA pathway contribution to formation of developmentally competent oocytes, which can support early development. Previously published data that were reanalysed here are available under accession codes GSE5241556, PRJNA471564, GSE7237958, GSE7489659, GSE11677160, GSE4941761, GSE13756362 and GSE5396063. The 103 third parties who use cookies on this service do so for their purposes of displaying and measuring personalized ads, generating audience insights, and developing and improving products. A UCSC browser snapshot shows a 3 Mb genomic region with upregulated retrotransposon loci (asterisks).Two RNA-seq libraries from hamster oocytes, which were outliers in PCA and had poor quality/low complexity (clearly because of a limited amount of starting material), were excluded from analysis.